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M9460706.TXT
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1994-06-25
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Document 0706
DOCN M9460706
TI Identification of a common clonal human immunodeficiency virus
integration site in human immunodeficiency virus-associated lymphomas.
DT 9408
AU Shiramizu B; Herndier BG; McGrath MS; Department of Pediatrics, San
Francisco General Hospital,; University of California 94110.
SO Cancer Res. 1994 Apr 15;54(8):2069-72. Unique Identifier : AIDSLINE
MED/94228518
AB Infection with human immunodeficiency virus type 1 (HIV-1) is associated
with a high incidence of lymphoma. Typically, the lymphomas are B-cell
in origin, and although they occur in the setting of HIV-1 infection,
historical studies have found no evidence for the presence of HIV-1
within the transformed B-cells. We describe a new class of large cell
lymphoma wherein HIV p24 expression within the tumor specimens was found
to be extremely high. In the first case, HIV was expressed in the
tumor-associated transformed T-cells. In three other cases, HIV was
found to be highly expressed in tumor-associated macrophages. These
tumors exhibited a mixed immunophenotype histologically. Analysis by
inverse polymerase chain reaction, using HIV long terminal repeat
primers, demonstrated monoclonal HIV integration sites for all four
tumors. Direct sequencing of the T-cell lymphoma inverse polymerase
chain reaction products identified the HIV integration site within the
fur gene, just upstream from the c-fes/fps protooncogene. Using segments
of the fur gene as a probe, the other three monoclonal integration sites
mapped to the same region. Although the integration and up-regulation of
c-fes/fps was localized to the tumor cells within the T-cell lymphoma,
the cells containing the monoclonal HIV in the other mixed
immunophenotype lymphomas are currently unknown. These observations
suggest that HIV may contribute directly to lymphomagenesis and identify
a common site of HIV integration within a subset of acquired
immunodeficiency syndrome lymphoma.
DE Base Sequence DNA Primers Human HIV-1/*GENETICS Immunophenotyping
Lymphoma, AIDS-Related/*GENETICS/IMMUNOLOGY/*MICROBIOLOGY/ PATHOLOGY
Lymphoma, B-Cell/GENETICS/MICROBIOLOGY/PATHOLOGY Molecular Sequence
Data Oligonucleotide Probes Polymerase Chain Reaction
Protein-Tyrosine Kinase/GENETICS Proto-Oncogene Proteins/GENETICS
*Proto-Oncogenes Restriction Mapping Support, Non-U.S. Gov't Support,
U.S. Gov't, P.H.S. *Virus Integration JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).